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KMID : 0613820170270101130
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Journal of Life Science
2017 Volume.27 No. 10 p.1130 ~ p.1136
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The Anti-inflammatory and Antiallergic Effects of Allomyrina dichotoma Larva Hot-water Extract
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Lee Hwa-Jeong
Seo Min-Chul
Kim In-Woo
Lee Joon-Ha
Hwang In-Yeong
Kim Mi-Ae
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Abstract
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Traditionally, the larvae of Allomyrina dichotoma (AD), a species of the rhinoceros beetle, have been widely used for their antidiabetic, antihepatofibrotic, antineoplastic, and antiobesity effects. The United Nations’ Food and Agriculture Organization has reported on the possibility of using edible insects in human dietary supplements in the future. However, despite the growing interest in insect-based bioactive products, the biological activities of these products have rarely been studied. Previously, we reported that AD larvae inhibit the in vitro differentiation of adipocytes via transcription factor downregulation. In this study, our objective was to evaluate the effects of a hot-water extract of AD larvae on allergy and inflammation. To investigate the inhibitory effect of the extract on allergic reactions, we measured the levels of β-hexosaminidase, tumor necrosis factor-α (TNF-α), interleukin-4 (IL-4), and cyclooxygenase-2 (COX-2) after activation of RBL-2H3 cells using Compound 48/80. In addition, the inhibitory effect of the extract on inflammation was determined using Raw 264.7 cells after lipopolysaccharide (LPS) stimulation. The extract significantly inhibited the β-hexosaminidase, TNF-α, IL-4, and COX-2 levels in RBL-2H3 cells. Furthermore, it effectively inhibited the inflammatory cytokine IL-6, nitric oxide, and inducible nitric oxide synthase expression in LPS-stimulated Raw 264.7 cells. These results suggest that AD larval extract can be potentially developed as an antiallergic and anti-inflammatory therapeutic agent.
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KEYWORD
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Allomyrina dichotoma (AD), anti-allergy, anti-inflammation, cytokine, nitric oxide
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